The attention to and demand for stronger linkages between research, policy and practice is increasing, especially in fields concerned with sensitive and challenging issues such as sexual and reproductive health and rights (SRHR) and HIV and AIDS. Share-Net International, the knowledge platform on SRHR, has been established to combine the expertise and strengths of Dutch organizations, Southern partners and key international actors working in the area of SRHR. To enhance Share-Net’s strategy for strengthening linkages between research, policy and practice, the present study aimed to obtain a better understanding of perceptions of knowledge within the Dutch fields of research, policy and practice involved with SRHR and HIV and AIDS in international development, how knowledge between these fields is linked and how these linkages can be strengthened.